Human Cytokeratin 14 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3164
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # LL001
Product Specifications
Immunogen
Human Cytokeratin 14 synthetic peptide
DGKVVSTHEQVLRTKN
DGKVVSTHEQVLRTKN
Specificity
Detects human Cytokeratin 14 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Cytokeratin 14 Antibody
Cytokeratin 14 in A431 Human Cell Line.
Cytokeratin 14 was detected in immersion fixed A431 human epithelial carcinoma cell line using Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (Catalog # MAB3164) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Cytokeratin 14 in NHEK Human Normal Epidermal Keratinocytes.
Cytokeratin 14 was detected in immersion fixed NHEK human normal epidermal keratinocytes using Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (Catalog # MAB3164) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007). CKAP4/p63 was also detected using Sheep Anti-Human CKAP4/p63 Affinity-purified Polyclonal Antibody (Catalog # AF7355) and stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green; Catalog # NL012). Cells were counterstained with DAPI (blue). Specific staining of Cytokeratin 14 was localized to intermediate filaments. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Cytokeratin 14 in human skin.
Cytokeratin 14 was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human Cytokeratin 14 Monoclonal Antibody (Catalog # MAB3164) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human Cytokeratin 14 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed A431 human epithelial carcinoma cell line and NHEK human normal epidermal keratinocytes
Sample: Immersion fixed A431 human epithelial carcinoma cell line and NHEK human normal epidermal keratinocytes
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin
Sample: Immersion fixed paraffin-embedded sections of human skin
Western Blot
Purkis, P. et al. (1990) J. Cell Sci. 97:39. This application was not tested by R&D Systems.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4 using MAB3164 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytokeratin 14
Additional Cytokeratin 14 Products
Product Documents for Human Cytokeratin 14 Antibody
Product Specific Notices for Human Cytokeratin 14 Antibody
For research use only
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