TcBuster™ Transposon CD19CAR-DHFR-eGFP

TcBuster Transposon CD19CAR-DHFR-eGFP Plasmid Map.

Primary human T cells edited with the TcBuster system express CD19CAR and eGFP. Primary human T cells were gene edited with TcBuster-M transposase (Catalog # TCB-001.1-100) and CD19CAR-DHFR-eGFP transposon (Catalog # TCBP001-100). On day 7 after gene editing, T cells were stained with Recombinant Human CD19 Protein, Atto 647N Conjugate (Catalog # ATM9269) and analyzed by flow cytometry for CD19CAR and eGFP expression. Representative flow cytometry plots of primary T cells edited with or without TcBuster-M transposase.

Mechanistic Diagram of the TcBuster Non-Viral Gene Editing System. The TcBuster system components are introduced into cells via electroporation (1). The TcBuster-M transposase mRNA is then translated into the transposase enzyme (2), which binds to the inverted terminal repeats (ITRs) on the DNA transposon (3). The transposase enzyme excises the genes of interest (GOI) from the transposon (4) and inserts them into the host genome (5). The GOI mRNA is transcribed from the host genome (6), and the protein is stably expressed in the edited cells (7).