TcBuster™ Transposon Insert On eGFP
R&D Systems, part of Bio-Techne | Catalog # TCBP002-100
Key Product Details
| Features: | TcBuster Transposon Insert On eGFP is a DNA transposon (plasmid) containing an eGFP sequence flanked by TcBuster-compatible ITR sequences that is only expressed after genomic integration. As a component of the TcBuster non-viral gene delivery system, this product requires TcBuster-M Transposase mRNA to function. This two component system can be introduced, by electroporation, into a wide variety of cell types to enable stable gene transfer. |
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TcBuster Transposon Insert On eGFP Plasmid Map. |
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HEK293E cells edited with the TcBuster system express eGFP. HEK293E cells were gene edited with TcBuster-M transposase (Catalog # TCB-001.1-100) and Insert-On-eGFP transposon (Catalog # TCBP002-100). On day 2 after gene editing, cells were analyzed by flow cytometry for expression of eGFP. Representative flow cytometry plots of HEK293E cells edited with or without TcBuster-M transposase. |
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CHO-K1 cells edited with the TcBuster system express eGFP. CHO-K1 cells were gene edited with TcBuster-M transposase (Catalog # TCB-001.1-100) and Insert-On-eGFP transposon (Catalog # TCBP002-100). On day 2 after gene editing, cells were analyzed by flow cytometry for expression of eGFP. Representative flow cytometry plots of CHO-K1 cells edited with or without TcBuster-M transposase. |
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