Trypsin 0.25% - EDTA 1mM (1X)

In cell culture research and cell biology studies enzymes are widely used to dissociate tissues and cell monolayers. The goal of cell isolation is to maximize the yield of functionally viable, dissociated cells. Despite the widespread use of enzymes for cell isolation applications over the years, very little is known about their mechanisms of action in cell dissociation and harvesting. Selection of the trypsin enzyme preparation of choice is dependent upon the type cells in culture. Trypsin solutions are enzyme preparations derived from porcine pancreas, generally used for the isolation and dissociation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary for the successful isolation of cells depends primarily on the cell type and the age of the culture.