When using MacCellect Cell Isolation kits (MAGM*** or MAGH***), why is it important to lyse red blood cells before starting the cell isolation protocol?

Name: MagCellect Mouse Naive CD8+ T Cell Isolation Kit
Brand: R&D Systems
SKU: MAGM207
Rating: 5 (1 reviews)

It is important to remove red blood cells before the isolation protocol using MagCellect isolation kits because not doing so can cause a poorer yield of selected cells. Red blood cells become sticky during the incubation protocols and may interfere with the interactions needed for removal of unwanted populations.

MagCellect Mouse Naive CD8+ T Cell Isolation Kit

R&D Systems, part of Bio-Techne | Catalog # MAGM207

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Citations (5)

Product Datasheet / COA / SDS

Summary for MagCellect Mouse Naive CD8+ T Cell Isolation Kit

CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes.

Product Specifications for MagCellect Mouse Naive CD8+ T Cell Isolation Kit

Species	Mouse
Source	N/A

Preparation & Storage

Shipping Conditions	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD8

Item	Value
Entrez Gene IDs	925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)
Alternate Names	CD8, CD8 antigen, alpha polypeptide (p32), CD8A, CD8a molecule, CD_antigen: CD8a, LEU2, Leu2 T-lymphocyte antigen, MAL, OKT8 T-cell antigen, T cell co-receptor, T-cell antigen Leu2, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, T8 T-cell antigen, p32

MagCellect Mouse Naive CD8+ T Cell Isolation Kit

R&D Systems, part of Bio-Techne | Catalog # MAGM207

Key Product Details

Summary for MagCellect Mouse Naive CD8+ T Cell Isolation Kit

Product Specifications for MagCellect Mouse Naive CD8+ T Cell Isolation Kit

Preparation & Storage

Background: CD8

FAQs for MagCellect Mouse Naive CD8+ T Cell Isolation Kit

Product Documents for MagCellect Mouse Naive CD8+ T Cell Isolation Kit

Product Datasheets

COA

Certificate of Analysis

SDS

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