Hana Cell Sorter and Single Cell Dispenser

How does the Hana set up time compare to FACS? The Hana Single Cell Dispenser offers walk-up usability. Hana can be set up and run, achieving single cell isolation in less than 7 minutes. Conventional FACS systems can take over an hour to set up and provide results.

How does the Hana Cell Sorter and Single Cell Dispenser compare to traditional FACS and limited dilution with sensitive cell lines? The Hana system is gentle and uses very low pressure (<2 psi) for cell sorting. In contrast, conventional FACS instruments use relatively high pressures (30-70 psi), which can be damaging for sensitive cells, resulting in reduced cell viability. The figure on the left shows a comparison of cellular stress response genes between cells isolated using a FACS instrument vs. using Bio-Techne's gentle cell sorting technology. Cells sorted with a FACS instrument showed induction of cellular stress response genes such as Ubiquitin C (Ubc), while cells sorted using Bio-Techne's technology show much lower expression of stress response genes. Limited dilution, on the other hand, is a gentle technique but does not yield high rates of single cell clones and has no fluorescence sorting capabilities resulting in laborious screening and high experimental failure rates.

Can Hana fit in a tissue culture hood to keep my samples sterile? Hana is a compact, benchtop system ideal for fitting into a tissue culture hood for sterile sorting. You can carry out all the preparatory work on your cells and isolate them at the same workstation! At 22 lbs., Hana can be moved outside of the hood when not in use to free up valuable space.

I run multiple samples and sterile isolation is important in my work, how does the Hana cell sorter mitigate contamination risk? Hana utilizes disposable microfluidic cell cartridges, which are specifically designed for sterile isolation. The closed cartridge design, and the ease of switching between cartridges with different samples, eliminates the potential for sample to sample carryover.

Hana is an innovative, easy-to-use system, supported by world class customer support. The Hana system is automated and easy-to-use with an intuitive software interface. There is no extensive training or specialized expertise required. If you’re not sure, call tech support and we’ll be happy to help!