Lightning-Link (R) PE-Cy5.5 Antibody Labeling Kit
Novus Biologicals, part of Bio-Techne | Catalog # 761-0005
Key Product Details
Applications
Flow Cytometry
Kit Type
Antibody Labeling Kit
Conjugate
PE/Cy5.5
Product Summary for Lightning-Link (R) PE-Cy5.5 Antibody Labeling Kit
Lightning-Link antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).
Our PE/Cy5.5 antibody labeling kit enables the direct conjugation of the PE/Cy5.5 tandem dye to any biomolecule with an available amine group. The researcher simply pipettes the antibody or other biomolecule into the vial of Lightning-Link label and incubates for 3 hours.
PE/Cy5.5 is a tandem conjugate. The PE has three maximal absorbance values of 498, 544 and 566nm with the optimal depending on the application. The PE functions as an energy donor for the Cy5.5. Energy is transferred from the PE to the Cy5.5 via energy resonance transfer. The Cy5.5 emits the energy received from the PE in the form of long wavelength light at 700nm.
Learn more about Lightning-Link™ Conjugation Kits by reading FAQs
For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay
Our PE/Cy5.5 antibody labeling kit enables the direct conjugation of the PE/Cy5.5 tandem dye to any biomolecule with an available amine group. The researcher simply pipettes the antibody or other biomolecule into the vial of Lightning-Link label and incubates for 3 hours.
| Features | Benefits |
| Quick and easy to use | Save time, no special knowledge required |
| No separation steps | 100% recovery - no antibody/protein loss |
| Can be used in a wide range of applications | Flexible |
| Freeze dried | Ships at ambient temperature, long shelf-life |
| Fully scalable (10 ug to 1 g or more) | Easy transfer from R&D to manufacturing |
| Stringently QC tested | Consistent high quality, excellent batch-to-batch reproducibility |
| Large number of labels available | Experimental flexibility |
| Reliable: nearly 300 references | Successfully used in many fields of research |
PE/Cy5.5 is a tandem conjugate. The PE has three maximal absorbance values of 498, 544 and 566nm with the optimal depending on the application. The PE functions as an energy donor for the Cy5.5. Energy is transferred from the PE to the Cy5.5 via energy resonance transfer. The Cy5.5 emits the energy received from the PE in the form of long wavelength light at 700nm.
Learn more about Lightning-Link™ Conjugation Kits by reading FAQs
For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay
Product Specifications
Application Notes
By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%. This kit can be used to label up to 60 ug of antibody, and is supplied in one vial.
Scientific Data Images for Lightning-Link (R) PE-Cy5.5 Antibody Labeling Kit
Flow Cytometry: Lightning-Link PE-Cy5.5 Antibody Labeling Kit [761-0005]
Kit Contents for Lightning-Link (R) PE-Cy5.5 Antibody Labeling Kit
- 1 or 3 glass vial(s) of Lightning-Link mix
- 1 vial of LL-Modifier reagent
- 1 vial of LL-Quencher reagent
Formulation, Preparation, and Storage
Concentration
Concentration is not relevant for this product. Please see the protocols for proper use of this product.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at -20C.
Background: Lightning-Link (R) PE-Cy5.5
The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.
Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates. As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.
Additional Lightning-Link (R) PE-Cy5.5 Products
Product Documents for Lightning-Link (R) PE-Cy5.5 Antibody Labeling Kit
Product Specific Notices for Lightning-Link (R) PE-Cy5.5 Antibody Labeling Kit
This product is manufactured by Abcam and distributed by Novus Biologicals.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.
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