Lightning-Link (R) PerCP-Cy5.5 Antibody Labeling Kit

Novus Biologicals, part of Bio-Techne | Catalog # 763-0015

Novus Biologicals, part of Bio-Techne
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Key Product Details

Applications

Flow Cytometry, Microscopy

Kit Type

Antibody Labeling Kit

Conjugate

PerCP/Cy5.5

Product Summary for Lightning-Link (R) PerCP-Cy5.5 Antibody Labeling Kit

Lightning-Link antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).

Our PerCP/Cy5.5 antibody labeling kit enables the direct conjugation of the PerCP/Cy5.5 tandem dye to any biomolecule with an available amine group. The researcher simply pipettes the antibody or other biomolecule into the vial of Lightning-Link label and incubates for 3 hours.

FeaturesBenefits
Quick and easy to useSave time, no special knowledge required
No separation steps100% recovery - no antibody/protein loss
Can be used in a wide range of applicationsFlexible
Freeze driedShips at ambient temperature, long shelf-life
Fully scalable (10 ug to 1 g or more)Easy transfer from R&D to manufacturing
Stringently QC testedConsistent high quality, excellent batch-to-batch reproducibility
Large number of labels available Experimental flexibility
Reliable: nearly 300 referencesSuccessfully used in many fields of research


PerCP/Cy5.5 is a tandem label. The PerCP excited at 484nm and functions as an energy donor for the Cy5.5. Energy is transferred from the PerCP to the Cy5.5 via energy resonance transfer. The Cy5.5 emits the energy received from the PerCP in the form of long wavelength light at 692nm.

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay

Product Specifications

Application Notes

PerCP/Cy5.5 is a tandem conjugate. In our Lightning-Link PerCP/Cy5.5 the best result is obtained when the tandem is excited at 488nm, wavelength at which the PerCP has its maximum absorption. The excited PerCP functions as an energy donor, where the energy is transferred via FRET (Fluorescence Resonance Energy Transfer) to the Cy5.5. The Cy5.5 emits then the energy received from the PE in the form of long wavelength light at 700nm. Use in FLOW cytometry reported in scientific literature ( PMID 26483406). This kit can be used to label up to 1 mg of antibody, and is supplied in one vial.

Scientific Data Images for Lightning-Link (R) PerCP-Cy5.5 Antibody Labeling Kit

Flow Cytometry: Lightning-Link (R) PerCP-Cy5.5 Antibody Labeling Kit [763-0015]

Flow Cytometry: Lightning-Link (R) PerCP-Cy5.5 Antibody Labeling Kit [763-0015]

Flow Cytometry: Lightning-Link PerCP-Cy5.5 Antibody Labeling Kit [763-0015] - Mouse anti-human CD3 was conjugated with PerCP/Cy5.5 using Lightning-Link kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).

Kit Contents for Lightning-Link (R) PerCP-Cy5.5 Antibody Labeling Kit

  • 1 or 3 glass vial(s) of Lightning-Link mix
  • 1 vial of LL-Modifier reagent
  • 1 vial of LL-Quencher reagent

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C.

Background: Lightning-Link (R) PerCP-Cy5.5

Lightning-Link® is an innovative technology that enables direct labeling of proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits.

The easy-to-use, one step procedure allows researchers to covalently label biomolecules with only 30 seconds hands on time. The researcher simply pipettes the biomolecule into a vial of lyophilized mixture containing the label of interest and incubates. Despite its apparent simplicity, the Lightning-Link® process is sophisticated and generates conjugates with performance characteristics identical to, or better than, those prepared with laborious multistep conjugation procedures.

The Lightning-Link® range currently consists of 50+ kits including enzymes, biotin, streptavidin and fluorochromes covering the spectrum from UV to far infrared. Lightning-Link® conjugates are used in a large number of scientific applications including Western Blotting, immunofluorescence, immunohistochemistry, flow cytometry, ELISA and FRET.

The technology is fully scalable (ug to grams) ensuring quality and consistency without any deterioration in the performance of the conjugate. As there are no separation or desalting steps, antibody recovery is 100%. Furthermore, the bond between the label and biomolecule is covalent, therefore the conjugate is extremely stable.

Additional Lightning-Link (R) PerCP-Cy5.5 Products

Product Documents for Lightning-Link (R) PerCP-Cy5.5 Antibody Labeling Kit

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lightning-Link (R) PerCP-Cy5.5 Antibody Labeling Kit

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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