Lightning-Link (R) R-PE Antibody Labeling Kit

Novus Biologicals, part of Bio-Techne | Catalog # 703-0004

Novus Biologicals, part of Bio-Techne
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Key Product Details

Applications

Flow Cytometry

Kit Type

Antibody Labeling Kit

Conjugate

R-Phycoerythrin

Product Summary for Lightning-Link (R) R-PE Antibody Labeling Kit

Lightning-Link antibody labeling kits enable the direct labeling of antibodies, proteins, peptides or other biomolecules for use in R&D applications, drug discovery and the development of diagnostic kits (See protocol for further information).

Our R-PE antibody labeling kit enables the direct conjugation of R-PE to any biomolecule with an available amine group. The researcher simply pipettes the antibody or other biomolecule into the vial of Lightning-Link R-PE and incubates for 3 hours.

FeaturesBenefits
Quick and easy to useSave time, no special knowledge required
No separation steps100% recovery - no antibody/protein loss
Can be used in a wide range of applicationsFlexible
Freeze driedShips at ambient temperature, long shelf-life
Fully scalable (10 ug to 1 g or more)Easy transfer from R&D to manufacturing
Stringently QC testedConsistent high quality, excellent batch-to-batch reproducibility
Large number of labels available Experimental flexibility
Reliable: nearly 300 referencesSuccessfully used in many fields of research


R-Phycoerythrin (R-PE) is a fluorescent protein from the phycobiliprotein family, present in red algae and cryptophytes. It has three maximal absorbance values of 498, 544 and 566nm (the optimal will depend on the application), and it has a strong emission peak at 580nm. RPE is closely related to B-Phycoerythrin (B-PE) and these are the most intense fluorescent phycobiliproteins providing an orange fluorescence.

Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay

Product Specifications

Application Notes

The recommended conjugation conditions are based on using a 1mg/ml antibody concentration and are designed to give a 1:1 Ab:RPE conjugation molar ratio. This kit can be used to label up to 3 mg of antibody, and is supplied in one vial.

Scientific Data Images for Lightning-Link (R) R-PE Antibody Labeling Kit

Flow Cytometry: Lightning-Link (R) R-PE Antibody Labeling Kit [703-0004]

Flow Cytometry: Lightning-Link (R) R-PE Antibody Labeling Kit [703-0004]

Flow Cytometry: Lightning-Link R-PE Antibody Labeling Kit [703-0004] - ndirect labeling with CD45RC supernatant (left). Indirect labeling with CD45RC purified antibody (middle). Direct labeling with Lightning-Link CD45RC-PE Purified antibody (left).
Lightning-Link R-PE Antibody Labeling Kit [703-0004] - Mouse anti-human CD8 was conjugated with R-Phycoerythrin using an Expedeon Lightning-Link kit. The conjugated antibody was then used to stain human peripheral blood lymphocytes, followed by analysis with flow cytometry. (Blue line - negative control; red line - positive staining).

Kit Contents for Lightning-Link (R) R-PE Antibody Labeling Kit

  • 1 or 3 or 5 glass vial(s) of Lightning-Link mix
  • 1 vial of LL-Modifier reagent
  • 1 vial of LL-Quencher reagent

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20C.

Background: Lightning-Link (R) R-PE

Easy R-PE labeling of your antibody with 30 seconds hands-on time. R-PE (R-Phycoerythrin) is a large 240kDa red phycobiliprotein isolated from red algae. R-PE has a strong absorption peak at 565nm and a secondary absorption peak at 492nm. The R-PE protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting. The Lightning-Link labeling system represents a quantum leap forward in antibody labeling technology. It allows the researcher to make labeled antibodies with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps. Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality labeled antibodies and proteins. As the procedure is not interrupted by desalting steps, trial labels can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.

Additional Lightning-Link (R) R-PE Products

Product Documents for Lightning-Link (R) R-PE Antibody Labeling Kit

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Lightning-Link (R) R-PE Antibody Labeling Kit

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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