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5-MethylCytosine ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62131

Novus Biologicals, part of Bio-Techne
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NBP2-62131

Key Product Details

Sample Type & Volume Required Per Well

DNA samples (100 uL)

Sensitivity

~0.5% 5-methylcytosine per 100ng single-stranded DNA

Assay Range

5-100% (100 ng/ul

Product Specifications

Assay Type

Indirect ELISA

Kit Type

ELISA Kit (Colorimetric)

Recovery for 5-MethylCytosine ELISA Kit (Colorimetric)

Recovery

N/A

Scientific Data Images for 5-MethylCytosine ELISA Kit (Colorimetric)

ELISA: 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131]

ELISA: 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131]

ELISA: Non-species specific 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131] - The workflow for the 5-Methylcytosine DNA ELISA kit utilizes the indirect ELISA methodology where denatured, single-stranded DNA (ssDNA) samples are coated on the plate well surfaces and a 5-mC mAb and conjugate HRP-Ab are added to the wells. Indirect det
ELISA: 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131]

ELISA: 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131]

ELISA: Non-species specific 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131] - Comparison of genomic DNA Mass Spectroscopy versus 5-MethylCytosine ELISA analysis. Genomic DNA samples include: human brain (HB), human kidney (HK), human embryonic stem cell (HESC), mouse brain (MB), mouse kidney (MK), and mouse testes (MT).
ELISA: 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131]

ELISA: 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131]

ELISA: Non-species specific 5-MethylCytosine ELISA Kit (Colorimetric) [NBP2-62131] - A typical standard curve for 5-MethylCytosine ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for 5-MethylCytosine ELISA Kit (Colorimetric)

  • Antibody-conjugate
  • Coating Buffer
  • Controls
  • ELISA Buffer
  • HRP Developer
  • Microtiter Plate
  • Primary Antibody

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: 5-MethylCytosine

Alternate Names

5-mC, 5-Methyl Cytosine, Cytosine (5-Methyl)

Additional 5-MethylCytosine Products

Product Documents for 5-MethylCytosine ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 5-MethylCytosine ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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