Human 5'-Nucleotidase/CD73 ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66370

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

37.50 pg/mL

Assay Range

62.50-4000 pg/mL

View all 5'-Nucleotidase/CD73 ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human 5-NT in samples. No significant cross-reactivity or interference between Human 5-NT and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 9.1%

Inter-Assay Precision (Precision between assays) CV% < 9.13%

Recovery for Human 5'-Nucleotidase/CD73 ELISA Kit (Chemiluminescence)

Recovery

85-107%

Linearity

Human 5'-Nucleotidase/CD73 ELISA Kit (Chemiluminescence)

Scientific Data Images for Human 5'-Nucleotidase/CD73 ELISA Kit (Chemiluminescence)

Human 5'-Nucleotidase/CD73 ELISA Kit (Chemiluminescence)

ELISA: Human 5'-Nucleotidase/CD73 ELISA Kit (Chemiluminescence) [NBP2-66370] -

ELISA: Human 5'-Nucleotidase/CD73 ELISA Kit (Chemiluminescence) [NBP2-66370] - Standard Curve Reference

Kit Contents for Human 5'-Nucleotidase/CD73 ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: 5'-Nucleotidase/CD73

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE

Gene Symbol

NT5E

Additional 5'-Nucleotidase/CD73 Products

Product Documents for Human 5'-Nucleotidase/CD73 ELISA Kit (Chemiluminescence)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human 5'-Nucleotidase/CD73 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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