Human ABCA1 ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66376
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 uL)
Sensitivity
0.10 ng/mL
Assay Range
0.16 - 10 ng/mL
Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Specificity
This kit recognizes Human ABCA1 in samples. No significant cross-reactivity or interference between human ABCA1 and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) CV% < 3.93%
Inter-Assay Precision (Precision between assays) CV% < 5.24%
Recovery for Human ABCA1 ELISA Kit (Colorimetric)
Recovery
90-110%
Linearity
Scientific Data Images for Human ABCA1 ELISA Kit (Colorimetric)
ELISA: Human ABCA1 ELISA Kit (Colorimetric) [NBP2-66376] -
ELISA: Human ABCA1 ELISA Kit (Colorimetric) [NBP2-66376] - Standard Curve ReferenceKit Contents for Human ABCA1 ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: ABCA1
ABCA1 is comprised of 2,261 amino acids with a theoretical molecular weight of 254 kDa and human ABCA1 shares 97% amino acid identity with mouse ABCA1. The general structure of ABCA consists of two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs). ABCA1 is a widely distributed cell-membrane protein with the highest expression found in macrophages. DHHC8 mediated palmitoylation of ABCA1 is essential for its localization to the plasma membrane and expression of mouse ABCA1 (not human) is induced by cAMP analogs (2). ABCA1 is phosphorylated at Ser1042 and Ser2054 by PKA, with Ser2054 being key for regulating phospholipid efflux. Mutations in ABCA1 have been linked to atherosclerosis and the progression of metabolic syndrome phenotypes: high density lipoprotein deficiency type 1 (HDLD1); also known as Tangier disease (TGD), and high density lipoprotein deficiency type 2 (HDLD2); also known as familial hypoalphalipoproteinemia (FHA) (3).
References
1.Oram JF, Lawn RM. (2001) ABCA1. The gatekeeper for eliminating excess tissue cholesterol. J Lipid Res. 42(8):1173-9. PMID: 11483617
2.Singaraja RR, Kang MH, Vaid K, Sanders SS, Vilas GL, Arstikaitis P, Coutinho J, Drisdel RC, El-Husseini Ael D, Green WN, Berthiaume L, Hayden MR. (2009) Palmitoylation of ATP-binding cassette transporter A1 is essential for its trafficking and function. Circ Res. 105(2):138-47. PMID: 19556522
3.Attie AD. (2007) ABCA1: at the nexus of cholesterol, HDL and atherosclerosis. Trends in Biochemical Sciences 32(4):172-9. PMID: 17324574
Long Name
ATP-binding Cassette, Sub-family A (ABC1), Member 1
Alternate Names
ABC1, CERP, HDLDT1, TGD
Gene Symbol
ABCA1
Additional ABCA1 Products
Product Documents for Human ABCA1 ELISA Kit (Colorimetric)
Product Specific Notices for Human ABCA1 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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