Human Actin Gamma 1 ELISA Kit (Chemiluminescence)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66399
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 uL)
Sensitivity
9.38 pg/mL
Assay Range
15.63-1000 pg/mL
Product Specifications
Assay Type
Sandwich-CLIA
Kit Type
ELISA Kit (Chemiluminescence)
Reactivity
Human
Specificity
This kit recognizes Human ACTG1 in samples. No significant cross-reactivity or interference between Human ACTG1 and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) CV% < 10.89%
Inter-Assay Precision (Precision between assays) CV% < 9.13%
Recovery for Human Actin Gamma 1 ELISA Kit (Chemiluminescence)
Recovery
89-110%
Linearity
Scientific Data Images for Human Actin Gamma 1 ELISA Kit (Chemiluminescence)
ELISA: Human Actin Gamma 1 ELISA Kit (Chemiluminescence) [NBP2-66399] -
ELISA: Human Actin Gamma 1 ELISA Kit (Chemiluminescence) [NBP2-66399] - Standard Curve ReferenceKit Contents for Human Actin Gamma 1 ELISA Kit (Chemiluminescence)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro CLIA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Substrate Reagent A
- Substrate Reagent B
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Actin Gamma 1
Actin Gamma 1 antibodies serve as excellent loading controls, due to the proteins' near ubiquitous expressn and strong conservation across most species. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Actin Gamma 1 antibody serves as an excellent loading control.
Alternate Names
ACT, ACTB, ACTG, actin, cytoplasmic 2, actin, gamma 1, cytoskeletal gamma-actin, deafness, autosomal dominant 20; deafness, autosomal dominant 26, DFNA20, DFNA26, Gamma-actin
Gene Symbol
ACTG1
Additional Actin Gamma 1 Products
Product Documents for Human Actin Gamma 1 ELISA Kit (Chemiluminescence)
Product Specific Notices for Human Actin Gamma 1 ELISA Kit (Chemiluminescence)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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