Human ADCY4 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66643

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.38 ng/mL

Assay Range

0.63 - 40 ng/mL

View all Adenylate Cyclase 4 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human ADCY4 in samples. No significant cross-reactivity or interference between Human ADCY4 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.92%

Inter-Assay Precision (Precision between assays) CV% < 5.08%

Recovery for Human ADCY4 ELISA Kit (Colorimetric)

Recovery

87-108%

Linearity

Human ADCY4 ELISA Kit (Colorimetric)

Scientific Data Images for Human ADCY4 ELISA Kit (Colorimetric)

Human ADCY4 ELISA Kit (Colorimetric)

ELISA: Human ADCY4 ELISA Kit (Colorimetric) [NBP2-66643] -

ELISA: Human ADCY4 ELISA Kit (Colorimetric) [NBP2-66643] - Standard Curve Reference

Kit Contents for Human ADCY4 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Adenylate Cyclase 4

Adenylyl cyclases function to convert ATP to cyclic AMP in response to activation by a variety of hormones, neurotransmitters and other regulatory molecules. Cyclic AMP, in turn, activates several other target molecules to control a broad range of diverse phenomena such as metabolism, gene transcription and memory. Adenylyl cyclases respond to receptor-initiated signals, mediated by the Gs and Gi heterotrimeric G proteins. The binding of an agonist to a Gs-coupled receptor catalyzes the exchange of GDP (bound to Galpha s) for GTP, the dissociation of GTP-Galpha s from Gbetagamma and Galpha s-mediated activation of adenylyl cyclase. Adenylyl cyclase IV (AC IV) and IX mRNA are expressed in all kidney nephron segments. AC IV exhibits moderate staining in type II and type IV fibrocytes in rat cochlea and immunoreactivity is also observed in type I fibrocytes. Activation of the D2 dopaminergic and m4 muscarine receptors inhibits the activity of adenylyl cyclase isozymes I, V, VI and VIII, whereas type II, IV and VII are stimulated and type III is not affected.

Long Name

Adenylate cyclase type 4

Alternate Names

ADCY4, Adenylyl cyclase 4

Gene Symbol

ADCY4

Additional Adenylate Cyclase 4 Products

Product Documents for Human ADCY4 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human ADCY4 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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