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Human Adenosine A2b R ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27186

Novus Biologicals, part of Bio-Techne
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NBP3-27186

Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates and other biological fluids (100 uL)

Sensitivity

0.061 ng/mL (example only; lot dependent)

Assay Range

0.156 - 10 ng/mL (example only; lot dependent)

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

Assay Length: 4.5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)

Scientific Data Images for Human Adenosine A2b R ELISA Kit (Colorimetric)

ELISA: Human Adenosine A2b R ELISA Kit (Colorimetric) [NBP3-27186] -

ELISA: Human Adenosine A2b R ELISA Kit (Colorimetric) [NBP3-27186] - Standard Curve Reference

Kit Contents for Human Adenosine A2b R ELISA Kit (Colorimetric)

  • Detection Reagent A
  • Detection Reagent B
  • Diluent Buffer
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Adenosine A2b R

The Adenosine A2b Receptor (ADORA2B) gene codes for a receptor located on chromosome 17 (near the Smith-Magenis syndrome region) that initiates adenylyl cyclase in the presence of adenosine. The encoded adenoside receptor is 332 amino acids long, around 36 kDA, and is member to the G protein-coupled receptor superfamily and interacts with netrin-1 to promote axon elongation. ADORA2B has been associated with cholera, hepatitis B, breast cancer, carcinoma, eclampsia, ischemia, asthma, cystic fibrosis, astrocytoma, and atherosclerosis. It is involved in signal transduction IP3 signaling, GPCR ligand binding and signaling, and the methotrexate pathway through interactions with the EZR, NTN1, ADA, DDC, and SLC9A3R2 genes.

Long Name

Adenosine A2b Receptor

Alternate Names

Adenosine A2bR, ADORA2B

Gene Symbol

ADORA2B

Additional Adenosine A2b R Products

Product Documents for Human Adenosine A2b R ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Adenosine A2b R ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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