Mouse Adenylate Kinase 1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68181

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.47 mIU/mL

Assay Range

0.78 - 50 mIU/mL

View all Adenylate Kinase 1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse AK1 in samples. No significant cross-reactivity or interference between Mouse AK1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.28%

Inter-Assay Precision (Precision between assays) CV% < 4.87%

Recovery for Mouse Adenylate Kinase 1 ELISA Kit (Colorimetric)

Recovery

84-104%

Linearity

Mouse Adenylate Kinase 1 ELISA Kit (Colorimetric)

Scientific Data Images for Mouse Adenylate Kinase 1 ELISA Kit (Colorimetric)

Mouse Adenylate Kinase 1 ELISA Kit (Colorimetric)

ELISA: Mouse Adenylate Kinase 1 ELISA Kit (Colorimetric) [NBP2-68181] -

ELISA: Mouse Adenylate Kinase 1 ELISA Kit (Colorimetric) [NBP2-68181] - Standard Curve Reference

Kit Contents for Mouse Adenylate Kinase 1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Adenylate Kinase 1

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

Long Name

Adenylate kinase isoenzyme 1

Alternate Names

AK 1, AK1, EC 2.7.4.3, EC 2.7.4.6, Myokinase

Gene Symbol

AK1

Additional Adenylate Kinase 1 Products

Product Documents for Mouse Adenylate Kinase 1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse Adenylate Kinase 1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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