Sheep Adiponectin/Acrp30 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-77004

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum and Plasma (50 - 100 uL)

Sensitivity

3.125 ng/mL

Assay Range

6.25 ng/mL - 100 ng/mL

View all Adiponectin/Acrp30 ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Sheep

Specificity

This assay has high sensitivity and excellent specificity for detection of Sheep Adiponectin/Acrp30. No significant cross-reactivity or interference between Sheep Adiponectin/Acrp30 and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 8

Inter-Assay Precision (Precision between assays) %CV < 10

Recovery for Sheep Adiponectin/Acrp30 ELISA Kit (Colorimetric)

Recovery

84 - 103%

Scientific Data Images for Sheep Adiponectin/Acrp30 ELISA Kit (Colorimetric)

Sheep Adiponectin/Acrp30 ELISA Kit (Colorimetric)

Sheep Adiponectin/Acrp30 ELISA Kit (Colorimetric) [NBP2-77004] -

Sheep Adiponectin/Acrp30 ELISA Kit (Colorimetric) [NBP2-77004] - Standard Curve Reference

Kit Contents for Sheep Adiponectin/Acrp30 ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay Plate
  • HRP-Conjugate (100 x concentrate)
  • HRP-Conjugate Diluent
  • Instruction Manual
  • Sample Diluent
  • Standard (10 x concentrate)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Adiponectin/Acrp30

Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.

Alternate Names

Acrp30, AdipoQ, ApM1, GBP28

Gene Symbol

ADIPOQ

Additional Adiponectin/Acrp30 Products

Product Documents for Sheep Adiponectin/Acrp30 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Sheep Adiponectin/Acrp30 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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