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Human AIF ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66673

Novus Biologicals, part of Bio-Techne
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NBP2-66673

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25-2000 pg/mL

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human AIF in samples. No significant cross-reactivity or interference between Human AIF and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 8.87%

Inter-Assay Precision (Precision between assays) CV% < 9.79%

Recovery for Human AIF ELISA Kit (Chemiluminescence)

Recovery

85-104%

Linearity

Human AIF ELISA Kit (Chemiluminescence)

Scientific Data Images for Human AIF ELISA Kit (Chemiluminescence)

Human AIF ELISA Kit (Chemiluminescence)

ELISA: Human AIF ELISA Kit (Chemiluminescence) [NBP2-66673] -

ELISA: Human AIF ELISA Kit (Chemiluminescence) [NBP2-66673] - Standard Curve Reference

Kit Contents for Human AIF ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: AIF

AIF, or apoptosis-inducing factor, is a flavoprotein localized to the mitochondrial membrane that plays a primary role in caspase-independent cell death, or apoptosis (1,2). AIF translocates to the nucleus when apoptosis is induced and causes mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. Apoptotic activity of AIF leads to chromatin condensation and DNA fragmentation (1). Similar to cytochrome c, AIF is a bifunctional protein with an oxidoreductase function and a second apoptogenic function. In contrast to cytochrome c, however, AIF acts in a caspase-independent fashion (2,3,4).

Long Name

Apoptosis Inducing Factor

Alternate Names

AIFM1, COXPD6, PDCD8

Gene Symbol

AIFM1

Additional AIF Products

Product Documents for Human AIF ELISA Kit (Chemiluminescence)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human AIF ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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