Rat Alpha Actinin 2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66419

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

View all alpha Actinin 2 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat ACTN2 in samples. No significant cross-reactivity or interference between Rat ACTN2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.19%

Inter-Assay Precision (Precision between assays) CV% < 5.4%

Recovery for Rat Alpha Actinin 2 ELISA Kit (Colorimetric)

Recovery

85-107%

Linearity

Rat Alpha Actinin 2 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Alpha Actinin 2 ELISA Kit (Colorimetric)

Rat Alpha Actinin 2 ELISA Kit (Colorimetric)

ELISA: Rat Alpha Actinin 2 ELISA Kit (Colorimetric) [NBP2-66419] -

ELISA: Rat Alpha Actinin 2 ELISA Kit (Colorimetric) [NBP2-66419] - Standard Curve Reference

Kit Contents for Rat Alpha Actinin 2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: alpha Actinin 2

Alpha actinin 2 is an actin binding proteins that carry out different purposes in different different cell types. Skeletal, cardiac, and smooth muscle isoforms are localized to the Z disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN2 encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. There are several known transcript variants that result from the use of multiple polyA+ sites. ACTN2 has the ability to form both homo- and heterodimers (with ACTN3).

Long Name

Actinin alpha 2

Alternate Names

Actinin alpha 2, Actinin-a2, ACTN2, alpha-Actinin 2, Alpha-Actinin Skeletal Muscle, Alpha-Actinin-2, CMD1AA

Gene Symbol

ACTN2

Additional alpha Actinin 2 Products

Product Documents for Rat Alpha Actinin 2 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Alpha Actinin 2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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