Human Alpha Actinin 4 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66421

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.47 ng/mL

Assay Range

0.78 - 50 ng/mL

View all Alpha Actinin 4 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human ACTN4 in samples. No significant cross-reactivity or interference between Human ACTN4 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.17%

Inter-Assay Precision (Precision between assays) CV% < 4.99%

Recovery for Human Alpha Actinin 4 ELISA Kit (Colorimetric)

Recovery

88-109%

Linearity

Human Alpha Actinin 4 ELISA Kit (Colorimetric)

Scientific Data Images for Human Alpha Actinin 4 ELISA Kit (Colorimetric)

Human Alpha Actinin 4 ELISA Kit (Colorimetric)

ELISA: Human Alpha Actinin 4 ELISA Kit (Colorimetric) [NBP2-66421] -

ELISA: Human Alpha Actinin 4 ELISA Kit (Colorimetric) [NBP2-66421] - Standard Curve Reference

Kit Contents for Human Alpha Actinin 4 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Alpha Actinin 4

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

Alternate Names

actinin alpha4 isoform, actinin, alpha 4, ACTININ-4, alpha-actinin-4, DKFZp686K23158, F-actin cross-linking protein, focal segmental glomerulosclerosis 1, FSGS, FSGS1, Non-muscle alpha-actinin 4

Gene Symbol

ACTN4

Additional Alpha Actinin 4 Products

Product Documents for Human Alpha Actinin 4 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Alpha Actinin 4 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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