alpha-Smooth Muscle Actin Antibody Pair

Novus Biologicals, part of Bio-Techne | Catalog # H00000059-AP41

Novus Biologicals, part of Bio-Techne
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Key Product Details

Assay Type

Sandwich ELISA

Assay Range

0.3 ng/ml-100 ng/ml

Reactivity

Human

View all alpha-Smooth Muscle Actin ELISA Kits »

Product Specifications

Description

Quality control test: Standard curve using recombinant protein (H00000059-P01) as an analyte. This antibody pair set comes with matched antibody pair to detect and quantify protein level of human ACTA2.

Conjugate

Unconjugated

Application Notes

Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.

Scientific Data Images for alpha-Smooth Muscle Actin Antibody Pair

ELISA: alpha-Smooth Muscle Actin Antibody Pair [H00000059-AP41]

ELISA: alpha-Smooth Muscle Actin Antibody Pair [H00000059-AP41]

ELISA: alpha-Smooth Muscle Actin Antibody Pair [H00000059-AP41] - Standard curve using human uterus myoma tissue lysate as an analyte (non-denatured). Sandwich ELISA detection sensitivity ranging from 100 ng/ml to 300 ug/ml for lysate total protein.
ELISA: alpha-Smooth Muscle Actin Antibody Pair [H00000059-AP41]

ELISA: alpha-Smooth Muscle Actin Antibody Pair [H00000059-AP41]

ELISA: alpha-Smooth Muscle Actin Antibody Pair [H00000059-AP41] - Standard curve using recombinant protein ( H00000059-P01 ) as an analyte. Sandwich ELISA detection sensitivity ranging from 0.3 ng/ml to 100 ng/ml.
Sandwich ELISA: alpha-Smooth Muscle Actin Antibody Pair [H00000059-AP41] - Detection sensitivity ranging from approximately 6561x to 729x dilution of the alpha-Smooth Muscle Actin 293T overexpression lysate (non-denatured). Standard curve using alpha-Smooth Muscle Actin 293T overexpression lysate (non-denatured) as an analyte.

Kit Contents for alpha-Smooth Muscle Actin Antibody Pair

  • Capture antibody: mouse monoclonal anti-ACTA2 IgG2b Kappa (100 ug)
  • Detection antibody: biotinylated mouse monoclonal anti-ACTA2 IgG1 Kappa (50 ug)

Preparation and Storage

Formulation

1x PBS, pH 7.4

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: alpha-Smooth Muscle Actin

Actin proteins are highly conserved in eukaryotic cells and are involved in cell motility, structure and integrity. Actin and myosin are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Actin filaments, along with microtubules, comprise a major component of the cytoskeleton and play essential roles in regulating cell shape and movement. Actin has been found to be expressed in at least six isoforms, each encoded by a separate gene and expressed in a developmentally regulated and tissue-specific manner. Alpha, beta and gamma actin isoforms have been identified. Alpha actins are a major constituent of the contractile apparatus, whereas beta and gamma actins are involved in the regulation of cell motility.

ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).

References

1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449

2 .El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G., . . . Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004

3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M., . . . Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084

Long Name

Actin, Alpha 2, Smooth Muscle, Aorta

Alternate Names

AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA

Gene Symbol

ACTA2

Additional alpha-Smooth Muscle Actin Products

Product Documents for alpha-Smooth Muscle Actin Antibody Pair

Product Datasheets

Certificate of Analysis

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Product Specific Notices for alpha-Smooth Muscle Actin Antibody Pair

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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