Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66423
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma and other biological fluids (100 uL)
Sensitivity
0.38 ng/mL
Assay Range
0.63-40 ng/mL
Product Specifications
Assay Type
Sandwich-CLIA
Kit Type
ELISA Kit (Chemiluminescence)
Reactivity
Human
Specificity
This kit recognizes Human ACT(alpha)2 in samples. No significant cross-reactivity or interference between Human ACT(alpha)2 and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) CV% < 9.77%
Inter-Assay Precision (Precision between assays) CV% < 7.69%
Recovery for Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence)
Recovery
86-114%
Linearity
Scientific Data Images for Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence)
ELISA: Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence) [NBP2-66423] -
ELISA: Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence) [NBP2-66423] - Standard Curve ReferenceKit Contents for Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence)
- Concentrated Biotinylated Detection Antibody (100X)
- Concentrated HRP Conjugate (100X)
- Concentrated Wash Buffer (25X)
- Detection Antibody Diluent
- HRP Conjugate Diluent
- Micro CLIA Plate(Dismountable)
- Plate Sealer
- Reference Standard
- Reference Standard & Sample Diluent
- Substrate Reagent A
- Substrate Reagent B
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: alpha-Smooth Muscle Actin
ACTA2 encodes alpha Smooth Muscle Actin and is also known as alpha-actin, alpha-actin-2, aortic smooth muscle or alpha smooth muscle actin (alpha-SMA, SMactin, alpha-SM-actin, ASMA). Alpha Smooth Muscle Actin is frequently used as a marker of smooth muscle differentiation and has a theoretical molecular weight of 42 kDa. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Expression of smooth muscle alpha actin is regulated by cell proliferation and is altered in pathological conditions including atherosclerosis and is common in metastatic cancers (1,3).
References
1. Chakraborty, R., Saddouk, F. Z., Carrao, A. C., Krause, D. S., Greif, D. M., & Martin, K. A. (2019). Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol, 39(4), 603-612. doi:10.1161/atvbaha.119.312449
2 .El Agha, E., Moiseenko, A., Kheirollahi, V., De Langhe, S., Crnkovic, S., Kwapiszewska, G., . . . Bellusci, S. (2017). Two-Way Conversion between Lipogenic and Myogenic Fibroblastic Phenotypes Marks the Progression and Resolution of Lung Fibrosis. Cell Stem Cell, 20(2), 261-273.e263. doi:10.1016/j.stem.2016.10.004
3. Chen, Y. C., Gonzalez, M. E., Burman, B., Zhao, X., Anwar, T., Tran, M., . . . Kleer, C. G. (2019). Mesenchymal Stem/Stromal Cell Engulfment Reveals Metastatic Advantage in Breast Cancer. Cell Rep, 27(13), 3916-3926.e3915. doi:10.1016/j.celrep.2019.05.084
Long Name
Actin, Alpha 2, Smooth Muscle, Aorta
Alternate Names
AAT6, ACTA2, Actin alpha 2, ACTSA, ACTVS, alphaSmooth Muscle Actin, MYMY5, SMA
Gene Symbol
ACTA2
Additional alpha-Smooth Muscle Actin Products
Product Documents for Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence)
Product Specific Notices for Human alpha-Smooth Muscle Actin ELISA Kit (Chemiluminescence)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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