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Mouse Aminomethyltransferase ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68202

Novus Biologicals, part of Bio-Techne
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NBP2-68202

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

46.88 pg/mL

Assay Range

78.13 - 5000 pg/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse AMT in samples. No significant cross-reactivity or interference between Mouse MAT and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.61%

Inter-Assay Precision (Precision between assays) CV% < 5.25%

Recovery for Mouse Aminomethyltransferase ELISA Kit (Colorimetric)

Recovery

85-100%

Linearity

Mouse Aminomethyltransferase ELISA Kit (Colorimetric)

Scientific Data Images for Mouse Aminomethyltransferase ELISA Kit (Colorimetric)

Mouse Aminomethyltransferase ELISA Kit (Colorimetric)

ELISA: Mouse Aminomethyltransferase ELISA Kit (Colorimetric) [NBP2-68202] -

ELISA: Mouse Aminomethyltransferase ELISA Kit (Colorimetric) [NBP2-68202] - Standard Curve Reference

Kit Contents for Mouse Aminomethyltransferase ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Aminomethyltransferase

The enzyme system for cleavage of glycine (glycine cleavage system; EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase; MIM 238300), H protein (a lipoic acid-containing protein; MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase; MIM 238331). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes.[supplied by OMIM]

Alternate Names

aminomethyltransferase, EC 2.1.2.10, GCE, GCSTaminomethyltransferase (glycine cleavage system protein T), glycine cleavage system protein T, NKHmitochondrial

Gene Symbol

AMT

Additional Aminomethyltransferase Products

Product Documents for Mouse Aminomethyltransferase ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse Aminomethyltransferase ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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