Human Angiopoietin-4 ELISA Kit (Chemiluminescence)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66730

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

18.75 pg/mL

Assay Range

31.25-2000 pg/mL

View all Angiopoietin-4 ELISA Kits »

Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human ANGPT4 in samples. No significant cross-reactivity or interference between Human ANGPT4 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 9.04%

Inter-Assay Precision (Precision between assays) CV% < 9.21%

Recovery for Human Angiopoietin-4 ELISA Kit (Chemiluminescence)

Recovery

89-116%

Linearity

Human Angiopoietin-4 ELISA Kit (Chemiluminescence)

Scientific Data Images for Human Angiopoietin-4 ELISA Kit (Chemiluminescence)

Human Angiopoietin-4 ELISA Kit (Chemiluminescence)

ELISA: Human Angiopoietin-4 ELISA Kit (Chemiluminescence) [NBP2-66730] -

ELISA: Human Angiopoietin-4 ELISA Kit (Chemiluminescence) [NBP2-66730] - Standard Curve Reference

Kit Contents for Human Angiopoietin-4 ELISA Kit (Chemiluminescence)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro CLIA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Substrate Reagent A
  • Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Angiopoietin-4

Angiopoietin 3 (ANG3), also called ANG4, might represent counterparts in mouse and human. Based on chromosomal analysis, mouse ANG3 is thought to be an ortholog of human ANG4. Human ANG4 acts as an agonist that binds and activates Tie2, whereas mouse ANG3 is likely to be a Tie2 antagonist, exerting agonist or antagonist activities depending on the cell context. Mouse ANG3 is expressed in various mouse tissues. Mouse angiopoietin 3 is a secreted glycoprotein and member of the angiopoietin family. It has an N terminal coiled-coil domain and a Cbterminal fibrinogen like domain. The coiled coil domains mediate ligand homo oligomerization and the fibrinogen like domains mediate ligand activity. Recombinant mouse angiopoietin 3 has a calculated molecular mass of 58 kDa. Due to glycosylation, the recombinant protein (containing 3 peptides) has an apparent molecular mass of 85-90 kDa in SDS-PAGE under reducing conditions. Mouse angiopoietin 3 shares 47%, 46%, and 54% sequence identity with mouse ANG1, mouse ANG2, and human ANG4, respectively.

Alternate Names

ANGPT4

Gene Symbol

ANGPT4

Additional Angiopoietin-4 Products

Product Documents for Human Angiopoietin-4 ELISA Kit (Chemiluminescence)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Angiopoietin-4 ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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