When using Rat Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (Colorimetric) NBP2-68215, what would be the dilution factor for wild type rat serum samples?

Name: Rat Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-68215

Our team diluted the samples 200-1000 fold and they have found the conentrations of Angiopoietin-like Protein 3/ANGPTL3 in rat serum to be in a range 85.2-498.5ng/ml. I have also checked other ELISA kits (with similar detection range) for mouse and human serum samples and in these they report diluting the samples 100 and 50 fold respectively.

Rat Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-68215

Submit a review

Product Datasheet / COA / SDS / Protocols & Manuals

100% Guarantee

Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

37.50 pg/mL

Assay Range

62.50 - 4000 pg/mL

View all Angiopoietin-like Protein 3/ANGPTL3 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat ANGPTL3 in samples. No significant cross-reactivity or interference between Rat ANGPTL3 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.09%

Inter-Assay Precision (Precision between assays) CV% < 5.09%

Recovery for Rat Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (Colorimetric)

Recovery

90-110%

Linearity

Scientific Data Images for Rat Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (Colorimetric)

ELISA: Rat Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (Colorimetric) [NBP2-68215] -

ELISA: Rat Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (Colorimetric) [NBP2-68215] - Standard Curve Reference

Kit Contents for Rat Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Angiopoietin-like Protein 3/ANGPTL3

ANGPTL3 is a 460 amino acid protein that is found most commonly in the liver, but has also been shown to reside in kidney samples, and contains three domains consisting of a signal peptide, N-terminal coiled-coil and C-terminal Fibrinogen (FBN) like domain. This protein is involved with the lipid metabolism disease pathways in relation to low levels of LDL (low density lipoproteins), HDL (High density lipoproteins), and triglycerides in plasma. This protein plays a role in angiogenesis and has been shown to interact with ITGAV, ITGB3, and ITGA5, and work is being done to study the protein and the related hypobetalipoproteinemia and atherosclerosis involvement.

Alternate Names

AGNPT5, Ang-5, Angiopoietin-5, ANGPTL3

Gene Symbol

ANGPTL3

Additional Angiopoietin-like Protein 3/ANGPTL3 Products

Product Documents for Rat Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (Colorimetric)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Rat Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (Colorimetric) Manual

COA

SDS

Download SDS

Product Specific Notices for Rat Angiopoietin-like Protein 3/ANGPTL3 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Citations
Reviews

Protocols
FAQs