Does APOE (Human) ELISA Kit KA1031 work in saliva?

Name: Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: KA1031

We do believe this kit APOE (Human) ELISA Kit KA1031 will work in saliva. We would recommend a titration for determination of the optimal dilutions since we have not made the determination.

Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1031

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Product Specifications
Precision
Recovery
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Supernatants, CSF, Plasma, Serum (50 uL)

Sensitivity

4.3 ng/mL

Assay Range

0.016 to 1 ug/mL

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

APOE (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human APOE.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.60%

Inter-Assay Precision (Precision between assays) CV% < 7.60%

Recovery for Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

Recovery

99.50%

Scientific Data Images for Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

ELISA: Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric) [KA1031]

ELISA: Apolipoprotein E/ApoE ELISA Kit [HRP] [KA1031] - Standard curve

Kit Contents for Human Apolipoprotein E/ApoE ELISA Kit (Colorimetric)

Biotinylated Human Apolipoprotein E Antibody (50x): A 50-fold concentrated biotinylated polyclonal antibody against human Apo-E.
Chromogen Substrate (1x): A stabilized peroxidase chromogen substrate tetramethylbenzidine.
Human Apolipoprotein E Microplate: A 96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against human Apo-E.
Human Apolipoprotein E Standard: Human Apo-E in a buffered protein base, lyophilized.
MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
Sealing Tapes: Pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay.
SP Conjugate (100x): A 100-fold concentrate.
Standard Diluent (1x): A buffered protein base with stabilizer.
Stop Solution (1x): A 0.5 N hydrochloric acid solution to stop the chromogen substrate reaction.
Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Apolipoprotein E/ApoE

Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.

The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.

Alternate Names

APOE

Gene Symbol