Human APP ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61301

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP2-61301
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Sample Type & Volume Required Per Well

Cell lysate, cerebral spinal fluid, plasma and serum samples (50 uL)

Sensitivity

0.92 pM

Assay Range

11.72 - 1500 pM

View all APP ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Precision

Intra-Assay Precision (Precision within an assay) Mean 2.5%

Inter-Assay Precision (Precision between assays) Mean 5.9%

Recovery for Human APP ELISA Kit (Colorimetric)

Recovery

Cell Lysate - 101.1%
Cerebrospinal Fluid - 93.6%

Scientific Data Images for Human APP ELISA Kit (Colorimetric)

ELISA: Human APP ELISA Kit (Colorimetric) [NBP2-61301]

ELISA: Human APP ELISA Kit (Colorimetric) [NBP2-61301]

ELISA: APP ELISA Kit (Colorimetric) [NBP2-61301] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ELISA: Human APP ELISA Kit (Colorimetric) [NBP2-61301]

ELISA: Human APP ELISA Kit (Colorimetric) [NBP2-61301]

ELISA: APP ELISA Kit (Colorimetric) [NBP2-61301] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ELISA: Human APP ELISA Kit (Colorimetric) [NBP2-61301]

ELISA: Human APP ELISA Kit (Colorimetric) [NBP2-61301]

ELISA: APP ELISA Kit (Colorimetric) [NBP2-61301] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Human APP ELISA Kit (Colorimetric)

  • Antibody-HRP-conjugate
  • Assay Buffer
  • Detector Antibody
  • Microtiter Plate
  • Standard
  • TMB Substrate
  • Wash Buffer Concentrate

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4°C.

Background: APP

Alzheimer's disease (AD) is a progressive neurodegenerative disease characterized by the senile plaques, neurofibrillary tangles and loss of synapses and neurons. AD has been largely viewed as a disease of toxicity being mediated by the accumulation of the amyloid beta (AB) peptide as plaques within the brain resulting in damage to brain cells from the binding of damaging metals, reactive oxygen species production and direct damage to cellular membranes. Recent research has suggested that the AB peptide is a multifunctional peptide with non-pathological effects and that its association with AD is in conjunction with its roles in combination with other proteins such as the amyloid precursor protein (APP) resulting in the imbalance between the processes of memory formation and normal forgetting. It is through the interactions of the AB peptide with APP that the AB peptide itself can affect normal modulation and signaling of APP resulting in its indicated role in the pathogenesis of AD via signaling effects rather than chemical or physical effects. There are three major APP isoforms (APP695, APP751 and APP770) that are formed through alternative splicing of precursor mRNA. APP770 represents the canonical sequence. The APP695 isoform is preferentially expressed in the central nervous system, while APP770 and APP751 are more highly expressed in peripheral tissues. It has been demonstrated that the full length APP695 can be cleaved via caspase at an intracellular site (Asp664) resulting in the release of a 31 amino acid C-terminal peptide (C31) from the remaining larger neo-APP fragment (APP C31) with both of these entities being pro-apoptotic. Immunohistochemical analysis of human brain tissue demonstrated that this cytoplasmic cleavage occurs 4-fold greater in patients with AD versus normal patients and that these cleavage products are localized to plaques and tangles in key areas of the brain affected by the disease. A single genetic mutation of aspartic acid residue 664 to alanine of APP695 led to the complete blockage of the C-terminal cleavage in vivo reversing many characteristics of the AD phenotype in a transgenic mouse model. Additionally, in cell culture it has been suggested that the neurotoxicity of AB is dependent on the cleavage of APP at Asp664 and the resulting AB-facilitated APP multimerization.

Long Name

Amyloid Precursor Protein

Alternate Names

Amyloid beta, beta Amyloid, Protease Nexin II

Gene Symbol

APP

Additional APP Products

Product Documents for Human APP ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human APP ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.