Rat BACE-2 ELISA Kit (Colorimetric)

Name: Rat BACE-2 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-69920

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69920

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

1.88 ng/mL

Assay Range

3.13 - 200 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat BACE2 in samples. No significant cross-reactivity or interference between Rat BACE2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.04%

Inter-Assay Precision (Precision between assays) CV% < 4.77%

Recovery for Rat BACE-2 ELISA Kit (Colorimetric)

Recovery

88-106%

Linearity

Scientific Data Images for Rat BACE-2 ELISA Kit (Colorimetric)

ELISA: Rat BACE-2 ELISA Kit (Colorimetric) [NBP2-69920] -

ELISA: Rat BACE-2 ELISA Kit (Colorimetric) [NBP2-69920] - Standard Curve Reference

Kit Contents for Rat BACE-2 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: BACE-2

BACE2 (Memapsin 1) is a membrane-bound aspartic protease that is highly homologous with BACE1 (Memapsin 2). While BACE1 processes the amyloid precursor protein (APP) at a key step in generating the beta-amyloid peptide and presumably causes Alzheimer's disease (AD), BACE2 has not been demonstrated to be directly involved in APP processing, and its physiological functions remain to be determined. In vivo, BACE2 is expressed as a precursor protein containing pre-, pro-, protease, transmembrane, and cytosolic domains/peptides.

Long Name

beta-site Ayloid Precursor Protein Cleaving Enzyme 2

Alternate Names

BACE2

Gene Symbol