Rat Bcl-2 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69947

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat Bcl-2 in samples. No significant cross-reactivity or interference between Rat Bcl-2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.92%

Inter-Assay Precision (Precision between assays) CV% < 5.33%

Recovery for Rat Bcl-2 ELISA Kit (Colorimetric)

Recovery

86-108%

Linearity

Rat Bcl-2 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Bcl-2 ELISA Kit (Colorimetric)

Rat Bcl-2 ELISA Kit (Colorimetric)

ELISA: Rat Bcl-2 ELISA Kit (Colorimetric) [NBP2-69947] -

ELISA: Rat Bcl-2 ELISA Kit (Colorimetric) [NBP2-69947] - Standard Curve Reference

Kit Contents for Rat Bcl-2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Bcl-2

The Bcl-2 family of proteins regulate apoptosis by controlling mitochondrial permeability and release of cytochrome c. Bcl-2 is an anti-apoptotic protein that resides in the outer mitochondrial wall and inhibits release of cytochrome c (1). Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies (2). It has also been shown that post-translational modification of Bcl-2, phosphorylation at serine 70, may be required to render its full and/or enhanced anti-apoptotic effect (3, 4).

Long Name

B Cell Lymphoma/Leukemia 2

Alternate Names

Bcl2

Gene Symbol

BCL2

Additional Bcl-2 Products

Product Documents for Rat Bcl-2 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Bcl-2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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