Human bcl-x ELISA Kit (Chemiluminescence)

Name: Human bcl-x ELISA Kit (Chemiluminescence)
Brand: Novus Biologicals
SKU: NBP2-69949

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69949

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

37.50 pg/mL

Assay Range

62.50-4000 pg/mL

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Product Specifications

Assay Type

Sandwich-CLIA

Kit Type

ELISA Kit (Chemiluminescence)

Reactivity

Human

Specificity

This kit recognizes Human BCL2L1/BCL-X in samples. No significant cross-reactivity or interference between Human BCL2L1/BCL-X and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 9.27%

Inter-Assay Precision (Precision between assays) CV% < 9.38%

Recovery for Human bcl-x ELISA Kit (Chemiluminescence)

Recovery

88-103%

Linearity

Scientific Data Images for Human bcl-x ELISA Kit (Chemiluminescence)

ELISA: Human bcl-x ELISA Kit (Chemiluminescence) [NBP2-69949] -

ELISA: Human bcl-x ELISA Kit (Chemiluminescence) [NBP2-69949] - Standard Curve Reference

Kit Contents for Human bcl-x ELISA Kit (Chemiluminescence)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro CLIA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Substrate Reagent A
Substrate Reagent B

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Bcl-x

Bcl-x is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Alternative splicing results in two distinct Bcl-x isoforms. Bcl-xL (long) is an anti-apoptotic protein that prevents release of Cytochromec from the mitochondrial intermembrane space into the cytosol. Bcl-xs (short) is a pro-apoptotic member that can initiate programmed cell death.

Long Name

B Cell Lymphoma/Leukemia x

Alternate Names

BCL2L1, Bclx

Gene Symbol

BCL2L1

Additional Bcl-x Products

Product Documents for Human bcl-x ELISA Kit (Chemiluminescence)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Human bcl-x ELISA Kit (Chemiluminescence) Manual

COA

Product Specific Notices for Human bcl-x ELISA Kit (Chemiluminescence)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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