Rat BMP-5 ELISA Kit (Colorimetric)

Name: Rat BMP-5 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-69996

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-69996

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat BMP-5 in samples. No significant cross-reactivity or interference between Rat BMP-5 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.34%

Inter-Assay Precision (Precision between assays) CV% < 4.93%

Recovery for Rat BMP-5 ELISA Kit (Colorimetric)

Recovery

92-108%

Linearity

Scientific Data Images for Rat BMP-5 ELISA Kit (Colorimetric)

ELISA: Rat BMP-5 ELISA Kit (Colorimetric) [NBP2-69996] -

ELISA: Rat BMP-5 ELISA Kit (Colorimetric) [NBP2-69996] - Standard Curve Reference

Kit Contents for Rat BMP-5 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: BMP-5

Bone morphogenic proteins (BMPs) are members of the TGFbeta superfamily. BMPs are involved in the induction of cartilage and bone formation. In vivo studies have shown that BMP-2 (also designated BMP-2A) and BMP-3 can independently induce cartilage formation. Smad3 association with the TGFbeta receptor complex and Smad1 translocation to the nucleus are observed after the addition of BMP-4 (also designated BMP-2B), suggesting that BMP-4 may play a role in activation of the Smad pathway. BMP-5, BMP-6 and BMP-7 all share high sequence homology with BMP-2, indicating that they each may be able to induce cartilage formation. BMP-8 (also designated OP-2) is thought to be involved in early development, as detectable expression has not been found in adult organs.

Long Name

Bone Morphogenetic Protein 5

Alternate Names

BMP5

Gene Symbol