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Human C1q ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA1274

Novus Biologicals, part of Bio-Techne
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KA1274

Key Product Details

Sample Type & Volume Required Per Well

Plasma, Serum (100 uL)

Sensitivity

0.5 U/mL

Assay Range

0-100 U/mL

Product Specifications

Assay Type

Indirect ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Description

C1q Ab ELISA Kit is an indirect solid phase enzyme immunoassay for the quantitative measurement of IgG class autoantibodies against C1q in human serum or plasma.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 3.18%

Inter-Assay Precision (Precision between assays) CV% < 3.07%

Kit Contents for Human C1q ELISA Kit (Colorimetric)

  • Calibrators A-F (0, 6.3, 12.5, 25, 50, 100 U/mL), containing C1q antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN3 0.09%), yellow. Ready to use.
  • Control positive (1) and negative (2), containing C1q antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN3 0.09%), yellow, Ready to use. The concentration is specified on the certificate of analysis.
  • Divisible microplate consisting of 12 modules of 8 wells each. Ready to use.
  • Enzyme conjugate containing anti-human IgG antibodies, HRP labelled; PBS, BSA, detergent, preservative ProClin 300 0.05%, light red. Ready to use.
  • Sample buffer P, containing PBS, BSA, detergent, preservative NaN3 0.09%, yellow, concentrate (5x).
  • Stop solution, contains acid. Ready to use.
  • TMB substrate, containing 3,3’,5,5’-Tetramethylbenzidin, colorless. Ready to use.
  • Wash solution, containing Tris, detergent, preservative NaN3 0.09%; 50x concentrated.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: C1q

C1q encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the A-chain polypeptide of human complement subcomponent C1q.

Alternate Names

A chain, complement component 1, q subcomponent, A chain, complement component 1, q subcomponent, alpha polypeptide

Gene Symbol

C1QA

Additional C1q Products

Product Documents for Human C1q ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human C1q ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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