For NBP2-74988, I would like to measure C1q in rat serum samples. I understand it is necessary to determine the optimal sample dilutions for my particular experiments myself, but if you have any information from when you validated rat serum samples, could you share the recommended dilution of normal rat serum samples?

Name: Rat C1q ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP2-74988

We have validated this kit with rat serum, and the samples were diluted 40000-80000 fold (200-800ug/mL).

Rat C1q ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74988

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.47 ng/mL

Assay Range

0.78 - 50 ng/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat C1 in samples. No significant cross-reactivity or interference between Rat C1 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.82%

Inter-Assay Precision (Precision between assays) CV% < 5.23%

Recovery for Rat C1q ELISA Kit (Colorimetric)

Recovery

88-106%

Linearity

Scientific Data Images for Rat C1q ELISA Kit (Colorimetric)

ELISA: Rat C1q ELISA Kit (Colorimetric) [NBP2-74988] -

ELISA: Rat C1q ELISA Kit (Colorimetric) [NBP2-74988] - Standard Curve Reference

Kit Contents for Rat C1q ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: C1q

C1q encodes a major constituent of the human complement subcomponent C1q. C1q associates with C1r and C1s in order to yield the first component of the serum complement system. Deficiency of C1q has been associated with lupus erythematosus and glomerulonephritis. C1q is composed of 18 polypeptide chains: six A-chains, six B-chains, and six C-chains. Each chain contains a collagen-like region located near the N terminus and a C-terminal globular region. The A-, B-, and C-chains are arranged in the order A-C-B on chromosome 1. This gene encodes the A-chain polypeptide of human complement subcomponent C1q.

Alternate Names

A chain, complement component 1, q subcomponent, A chain, complement component 1, q subcomponent, alpha polypeptide

Gene Symbol

C1QA

Additional C1q Products

Product Documents for Rat C1q ELISA Kit (Colorimetric)

Product Datasheets

Download Product Datasheets

Protocols & Manuals

Download Rat C1q ELISA Kit (Colorimetric) Manual

COA

SDS

Download SDS

Product Specific Notices for Rat C1q ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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