Rat Caldesmon/CALD1 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74970

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

View all Caldesmon/CALD1 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat CALD in samples. No significant cross-reactivity or interference between Rat CALD and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.41%

Inter-Assay Precision (Precision between assays) CV% < 4.57%

Recovery for Rat Caldesmon/CALD1 ELISA Kit (Colorimetric)

Recovery

86-109%

Linearity

Rat Caldesmon/CALD1 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Caldesmon/CALD1 ELISA Kit (Colorimetric)

Rat Caldesmon/CALD1 ELISA Kit (Colorimetric)

ELISA: Rat Caldesmon/CALD1 ELISA Kit (Colorimetric) [NBP2-74970] -

ELISA: Rat Caldesmon/CALD1 ELISA Kit (Colorimetric) [NBP2-74970] - Standard Curve Reference

Kit Contents for Rat Caldesmon/CALD1 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Caldesmon/CALD1

Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin (1,2). Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus (3). Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin (4).

Alternate Names

CALD1, CDM, HCAD, LCAD, NAG22

Gene Symbol

CALD1

Additional Caldesmon/CALD1 Products

Product Documents for Rat Caldesmon/CALD1 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Caldesmon/CALD1 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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