Rat Calreticulin ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06821
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma, cell supernatant, and other biological fluids (100 ul)
Sensitivity
37.5 pg/mL
Assay Range
62.5 - 4000 pg/mL
Product Specifications
Assay Type
Sandwich-ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Rat
Specificity
Rat Calreticulin ELISA Kit (Colorimetric) recognizes Rat Calreticulin in samples. No significant cross-reactivity or interference between Rat Calreticulin and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) 4.89
Inter-Assay Precision (Precision between assays) 4.94
Recovery for Rat Calreticulin ELISA Kit (Colorimetric)
Recovery
83 - 106 %
Linearity
Scientific Data Images for Rat Calreticulin ELISA Kit (Colorimetric)
ELISA: Rat Calreticulin ELISA Kit (Colorimetric) [NBP3-06821] -
ELISA: Rat Calreticulin ELISA Kit (Colorimetric) [NBP3-06821] - Standard Curve ReferenceKit Contents for Rat Calreticulin ELISA Kit (Colorimetric)
- Biotinylated Detection Ab Diluent
- Concentrated Biotinylated Detection Ab (100x)
- Concentrated HRP Conjugate (100x)
- Concentrated Wash Buffer (25x)
- HRP Conjugate Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Calreticulin
Given its role in multiple biological processes, it makes sense that calreticulin is implicated in both healthy and disease states. Studies have found that calreticulin mutations were present in patients with myeloproliferative neoplasms (MFN) and essential thrombocythaemia (ET) (4). Calreticulin expression is typically upregulated in most cancer lines, however it is downregulated in some tissues including cervical carcinomas, prostate cancer, and human colon adenocarcinoma (3). High expression of calreticulin on cancer cells is related to tumor cell phagocytosis and is often correlated with, and counteracted by, elevated CD47 expression to prevent cancer cell phagocytosis (3). Calreticulin mutations can serve as a major diagnostic biomarker for MFN and ET and additionally the calreticulin gene may be a potential target for cancer therapeutics (3,4).
References
1. Michalak, M., Groenendyk, J., Szabo, E., Gold, L. I., & Opas, M. (2009). Calreticulin, a multi-process calcium-buffering chaperone of the endoplasmic reticulum. The Biochemical Journal. https://doi.org/10.1042/BJ20081847
2. Fucikova, J., Spisek, R., Kroemer, G., & Galluzzi, L. (2021). Calreticulin and cancer. Cell Research. https://doi.org/10.1038/s41422-020-0383-9
3. Sun, J., Mu, H., Dai, K., & Yi, L. (2017). Calreticulin: a potential anti-cancer therapeutic target. Die Pharmazie. https://doi.org/10.1691/ph.2017.7031
4. Prins, D., Gonzalez Arias, C., Klampfl, T., Grinfeld, J., & Green, A. R. (2020). Mutant Calreticulin in the Myeloproliferative Neoplasms. HemaSphere. https://doi.org/10.1097/HS9.0000000000000333
Alternate Names
Autoantigen Ro, CALR, Calregulin, cC1qR, CRT, SSA, Vasostatin
Gene Symbol
CALR
Additional Calreticulin Products
Product Documents for Rat Calreticulin ELISA Kit (Colorimetric)
Product Specific Notices for Rat Calreticulin ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
Loading...
Loading...
Loading...
Loading...