Canine VEGF Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # CAVE00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
Sensitivity
9.8 pg/mL (Cell Culture Supernates)
19.5 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
19.5 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Assay Range
19.5-1250 pg/mL (Cell Culture Supernates)
39.1-2500 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
39.1-2500 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Canine VEGF Quantikine ELISA Kit
The Quantikine Canine VEGF Immunoassay is a 4.5 hour solid phase ELISA designed to measure VEGF levels in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant canine VEGF and antibodies raised against the recombinant protein. Results obtained for naturally occurring canine VEGF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine VEGF.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Canine
Specificity
Natural and recombinant canine VEGF. This assay cross-reacts with Human VEGF165.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 29.1 | 123 | 531 | 32.8 | 128 | 495 |
| Standard Deviation | 1.9 | 5.0 | 18.4 | 2.8 | 6.4 | 33.0 |
| CV% | 6.5 | 4.1 | 3.5 | 8.5 | 5.0 | 6.7 |
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 53.7 | 235 | 910 | 64.5 | 250 | 1003 |
| Standard Deviation | 3.6 | 10.6 | 46.2 | 5.7 | 17.4 | 61.7 |
| CV% | 6.7 | 4.5 | 5.1 | 8.8 | 7.0 | 6.2 |
Recovery for Canine VEGF Quantikine ELISA Kit
The recovery of canine VEGF spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 102 | 94-110 |
| EDTA Plasma (n=4) | 98 | 92-107 |
| Heparin Plasma (n=4) | 107 | 96-118 |
| Serum (n=4) | 103 | 87-113 |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine VEGF and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Canine VEGF Quantikine ELISA Kit
Canine VEGF ELISA Cell Culture Supernate Standard Curve
Canine VEGF ELISA Serum/Plasma Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: VEGF
Long Name
Vascular Endothelial Growth Factor
Alternate Names
MVCD1, VAS, Vasculotropin, VEGF-A, VEGFA, VPF
Entrez Gene IDs
Gene Symbol
VEGFA
Additional VEGF Products
Product Documents for Canine VEGF Quantikine ELISA Kit
Product Specific Notices for Canine VEGF Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.