Rat Caspase-12 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75020

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.94 ng/mL

Assay Range

1.56 - 100 ng/mL

View all Caspase-12 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat CASP12 in samples. No significant cross-reactivity or interference between Rat CASP12 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.67%

Inter-Assay Precision (Precision between assays) CV% < 5.21%

Recovery for Rat Caspase-12 ELISA Kit (Colorimetric)

Recovery

86-102%

Linearity

Rat Caspase-12 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Caspase-12 ELISA Kit (Colorimetric)

Rat Caspase-12 ELISA Kit (Colorimetric)

ELISA: Rat Caspase-12 ELISA Kit (Colorimetric) [NBP2-75020] -

ELISA: Rat Caspase-12 ELISA Kit (Colorimetric) [NBP2-75020] - Standard Curve Reference

Kit Contents for Rat Caspase-12 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Caspase-12

Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene, CASP12, is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. (provided by RefSeq)

Alternate Names

CASP12, Caspase12

Gene Symbol

CASP12

Additional Caspase-12 Products

Product Documents for Rat Caspase-12 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Caspase-12 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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