Rat Caspase-4 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75027

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

1.88 ng/mL

Assay Range

3.13 - 200 ng/mL

View all Caspase-4 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat CASP4 in samples. No significant cross-reactivity or interference between Rat CASP4 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.18%

Inter-Assay Precision (Precision between assays) CV% < 5.02%

Recovery for Rat Caspase-4 ELISA Kit (Colorimetric)

Recovery

87-111%

Linearity

Rat Caspase-4 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Caspase-4 ELISA Kit (Colorimetric)

Rat Caspase-4 ELISA Kit (Colorimetric)

ELISA: Rat Caspase-4 ELISA Kit (Colorimetric) [NBP2-75027] -

ELISA: Rat Caspase-4 ELISA Kit (Colorimetric) [NBP2-75027] - Standard Curve Reference

Kit Contents for Rat Caspase-4 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Caspase-4

Caspase 4 encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.

Alternate Names

CASP4, Caspase4, ICE(rel)II, ICH-2, Mih1/TX, TX

Gene Symbol

CASP4

Additional Caspase-4 Products

Product Documents for Rat Caspase-4 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Caspase-4 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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