Human Caspase-8 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-83733

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, cell lysate, serum (50 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10.0 ng/mL

View all Caspase-8 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit detect both forms, the pro- as well as the active form of Caspase-8.

Description

The human Caspase-8 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Caspase-8

Precision

Intra-Assay Precision (Precision within an assay) CV% < 6.7%

Inter-Assay Precision (Precision between assays) CV% < 8.5%

Recovery for Human Caspase-8 ELISA Kit (Colorimetric)

Recovery

89.5%

Scientific Data Images for Human Caspase-8 ELISA Kit (Colorimetric)

ELISA: Human Caspase-8 ELISA Kit (Colorimetric) [NBP1-83733]

ELISA: Human Caspase-8 ELISA Kit (Colorimetric) [NBP1-83733]

ELISA: Caspase-8 ELISA Kit [NBP1-83733] - Standard curve

Kit Contents for Human Caspase-8 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Caspase-8
  • anti-human Caspase-8 polyclonal (rabbit) Detection Antibody
  • Anti-rabbit-IgG-HRP
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Human Caspase-8 Standard lyophilized, 20.00 ng/ml upon reconstitution
  • Lysis Buffer 10x
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Caspase-8

Caspases are the executioners of apoptosis. These cysteine protease family consists of more than 10 related members characterized by almost absolute specificity for aspartic acid in the P1 position. Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide together with one large and one small subunit. Activation of caspases during apoptosis results in the cleavage of critical cellular substrates so precipitating the dramatic morphological changes of apoptosis. Caspase-8 has been reported to have additional functions unrelated to cell death. It is required for T-cell homeostasis, proliferation, and cell activation.

Alternate Names

CASP8, Caspase8, Mch5

Gene Symbol

CASP8

Additional Caspase-8 Products

Product Documents for Human Caspase-8 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Caspase-8 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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