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Rat Cathepsin B ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-67255

Novus Biologicals, part of Bio-Techne
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NBP2-67255

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

0.10 ng/mL

Assay Range

0.16 - 10 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat CTSB in samples. No significant cross-reactivity or interference between Rat CTSB and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.78%

Inter-Assay Precision (Precision between assays) CV% < 4.72%

Recovery for Rat Cathepsin B ELISA Kit (Colorimetric)

Recovery

87-105%

Linearity

Rat Cathepsin B ELISA Kit (Colorimetric)

Scientific Data Images for Rat Cathepsin B ELISA Kit (Colorimetric)

Rat Cathepsin B ELISA Kit (Colorimetric)

ELISA: Rat Cathepsin B ELISA Kit (Colorimetric) [NBP2-67255] -

ELISA: Rat Cathepsin B ELISA Kit (Colorimetric) [NBP2-67255] - Standard Curve Reference

Kit Contents for Rat Cathepsin B ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Cathepsin B

The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S and O. The aspartyl protease class is composed of cathepsins D and E. cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. cathepsin B is expressed in luminal epithelial cells, indicating that cathepsin B is a marker for secretory cell death.

Alternate Names

CTSB

Gene Symbol

CTSB

Additional Cathepsin B Products

Product Documents for Rat Cathepsin B ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Cathepsin B ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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