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Mouse Cathepsin L ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89172

Novus Biologicals, part of Bio-Techne
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NBP2-89172

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

18.75 pg/mL

Assay Range

31.25-2000 pg/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse CTSL in samples. No Significant cross-reactivity or interference between Mouse CTSL and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 410

Inter-Assay Precision (Precision between assays) CV% < 43.4

Recovery for Mouse Cathepsin L ELISA Kit (Colorimetric)

Recovery

89-105%

Linearity

Mouse Cathepsin L ELISA Kit (Colorimetric)

Scientific Data Images for Mouse Cathepsin L ELISA Kit (Colorimetric)

Mouse Cathepsin L ELISA Kit (Colorimetric)

ELISA: Mouse Cathepsin L ELISA Kit (Colorimetric) [NBP2-89172] -

ELISA: Mouse Cathepsin L ELISA Kit (Colorimetric) [NBP2-89172] - Standard Curve Reference

Kit Contents for Mouse Cathepsin L ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Cathepsin L

Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumour invasion and metastasis. Normally located in lysosomes of almost all mammalian cells, they can be secreted from the cell under certain conditions. Cathepsin L is responsible for most of the intralysosomal breakdown of normal cells. Cathepsin L is secreted by numerous transformed cells in its inactive proform, and mRNA expression level seems to correlate with their metastatic potential.

Alternate Names

CTSL, CTSL1

Gene Symbol

CTSL

Additional Cathepsin L Products

Product Documents for Mouse Cathepsin L ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Mouse Cathepsin L ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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