Rat Cathepsin L ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89173

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.38 ng/mL

Assay Range

0.63-40 ng/mL

View all Cathepsin L ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat CTSL in samples. No Significant cross-reactivity or interference between Rat CTSL and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 7.35

Inter-Assay Precision (Precision between assays) CV% < 15.77

Recovery for Rat Cathepsin L ELISA Kit (Colorimetric)

Recovery

88-107%

Linearity

Rat Cathepsin L ELISA Kit (Colorimetric)

Scientific Data Images for Rat Cathepsin L ELISA Kit (Colorimetric)

Rat Cathepsin L ELISA Kit (Colorimetric)

ELISA: Rat Cathepsin L ELISA Kit (Colorimetric) [NBP2-89173] -

ELISA: Rat Cathepsin L ELISA Kit (Colorimetric) [NBP2-89173] - Standard Curve Reference

Kit Contents for Rat Cathepsin L ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Cathepsin L

Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumour invasion and metastasis. Normally located in lysosomes of almost all mammalian cells, they can be secreted from the cell under certain conditions. Cathepsin L is responsible for most of the intralysosomal breakdown of normal cells. Cathepsin L is secreted by numerous transformed cells in its inactive proform, and mRNA expression level seems to correlate with their metastatic potential.

Alternate Names

CTSL, CTSL1

Gene Symbol

CTSL

Additional Cathepsin L Products

Product Documents for Rat Cathepsin L ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Cathepsin L ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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