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Rat CD25/IL-2R alpha ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06789

Novus Biologicals, part of Bio-Techne
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NBP3-06789

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

0.19 ng/mL

Assay Range

0.31 - 20 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat CD25/IL-2R alpha in samples. No significant cross-reactivity or interference between Rat CD25/IL-2R alpha and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 5.69

Inter-Assay Precision (Precision between assays) 5.22

Recovery for Rat CD25/IL-2R alpha ELISA Kit (Colorimetric)

Recovery

83 - 106 %

Linearity

Rat CD25/IL-2R alpha ELISA Kit (Colorimetric)

Scientific Data Images for Rat CD25/IL-2R alpha ELISA Kit (Colorimetric)

Rat CD25/IL-2R alpha ELISA Kit (Colorimetric)

ELISA: Rat CD25/IL-2R alpha ELISA Kit (Colorimetric) [NBP3-06789] -

ELISA: Rat CD25/IL-2R alpha ELISA Kit (Colorimetric) [NBP3-06789] - Standard Curve Reference

Kit Contents for Rat CD25/IL-2R alpha ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CD25/IL-2R alpha

The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.(provided by RefSeq)

Long Name

Interleukin 2 Receptor alpha

Alternate Names

CD25, IL-2 R alpha, IL-2Ra, IL2R alpha, IL2RA

Gene Symbol

IL2RA

Additional CD25/IL-2R alpha Products

Product Documents for Rat CD25/IL-2R alpha ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat CD25/IL-2R alpha ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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