Human CD30/TNFRSF8 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91185

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution (25 uL)

Sensitivity

0.3 ng/mL

Assay Range

1.6 - 100 ng/mL

View all CD30/TNFRSF8 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human CD30 positive serum

Description

The human sCD30 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD30

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.10%

Inter-Assay Precision (Precision between assays) CV% < 5.60%

Recovery for Human CD30/TNFRSF8 ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 84% to 98% with an overall mean recovery of 92%. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Human CD30/TNFRSF8 ELISA Kit (Colorimetric)

Human CD30/TNFRSF8 ELISA Kit [NBP1-91185] - Data for Human sCD30 Platinum ELISA

Kit Contents for Human CD30/TNFRSF8 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD30
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Blue-Dye
  • Control high, lyophilized
  • Control low, lyophilized
  • Green-Dye
  • HRP-Conjugate anti-human sCD30 monoclonal antibody
  • Human sCD30 Standard lyophilized, 200 ng/ml upon reconstitution
  • Sample Diluent
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CD30/TNFRSF8

Characterization of the CD30 antigen has shown it to be in its mature form a transmembrane protein of about 120kDa elaborated from an 84kD cytoplasmic precursor primarily through glycosylation. The CD30 ligand (CD30L) has been identified, showing significant homology to TNF-alpha, TNF-beta, FasL, CD40L, CD27L and 4-1BBl. CD30L is expressed on activated T-cells. Interactions of the cytokine receptor CD30 with its ligand induces pleiotropic biologic effects, such as differentiation, activation, proliferation and cell death. CD30 expression is strictly dependent on activation and proliferation of T- and B-cells. In pathological conditions, CD30 positivity is regarded as a peculiar attribute of Hodgkin's and Reed-Sternberg cells. An 85kDa soluble form of the CD30 molecule (sCD30) has been shown to be released by CD30+ cell in vitro and in vivo. Increased serum levels of sCD30 have been reported for patients with CD30+ ALCL and CD30+ embryonal carcinoma of the testis and were found to correlate with the clinical phase of the disease, i.e. presentation complete remission (CR), relapse.

Alternate Names

CD30, TNFRSF8

Gene Symbol

TNFRSF8

Additional CD30/TNFRSF8 Products

Product Documents for Human CD30/TNFRSF8 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human CD30/TNFRSF8 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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