Human CD31/PECAM-1 ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91175
Key Product Details
Sample Type & Volume Required Per Well
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), urine, other body fluids (10 uL)
Sensitivity
0.06 ng/mL
Assay Range
0.47 - 30.0 ng/mL
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Specificity
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human CD31/PECAM-1 positive serum. There was no crossreactivity detected.
Description
The human sCD31/PECAM-1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD31/PECAM-1
Precision
Intra-Assay Precision (Precision within an assay) CV% < 1.70%
Inter-Assay Precision (Precision between assays) CV% < 7.40%
Recovery for Human CD31/PECAM-1 ELISA Kit (Colorimetric)
Recovery
The recovery ranged from 100% to 119% with an overall mean recovery of 108%. Please see protocol for more specific Spike Recovery details.
Scientific Data Images for Human CD31/PECAM-1 ELISA Kit (Colorimetric)
N/A: Human, CD31/PECAM-1 ELISA Kit (Colorimetric) [NBP1-91175] - Standard curve for human Human CD31/PECAM-1 ELISA Kit (Colorimetric)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: CD31/PECAM-1
PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.
References
1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial
2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3
3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9
Long Name
Platelet Endothelial Cell Adhesion Molecule 1
Alternate Names
CD31, EndoCAM, PECA1, PECAM-1, PECAM1
Gene Symbol
PECAM1
Additional CD31/PECAM-1 Products
Product Documents for Human CD31/PECAM-1 ELISA Kit (Colorimetric)
Product Specific Notices for Human CD31/PECAM-1 ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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