Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91184

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution (20 uL)

Sensitivity

0.06 ng/mL

Assay Range

0.16 - 10.0 ng/mL

View all CD40 Ligand/TNFSF5 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The assay detects both natural and recombinant human CD40 Ligand/TNFSF5. The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human CD40 Ligand/TNFSF5 positive sample. There was no cross reactivity or interference.

Description

The human sCD40L ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD40L

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.00%

Inter-Assay Precision (Precision between assays) CV% < 6.80%

Recovery for Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

Recovery

91%

Scientific Data Images for Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

ELISA: Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric) [NBP1-91184]

ELISA: Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric) [NBP1-91184]

ELISA: CD40 Ligand/TNFSF5 ELISA Kit [NBP1-91184] - Standard curve

Kit Contents for Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD40L
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Blue-Dye
  • Control high, lyophilized
  • Control low, lyophilized
  • Dilution Plates
  • Green-Dye
  • HRP-Conjugate anti-human sCD40L monoclonal antibody
  • Human sCD40L Standard lyophilized, 20 ng/ml upon reconstitution
  • Sample Diluent
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CD40 Ligand/TNFSF5

CD40 belongs to the TNF receptor superfamily and is a biochemical marker for the identification of activated platelets of patients with cardiovascular diseases (angina pectoris, infarction, stroke etc.) A key role of CD40/CD40ligand interactions in immune activation, particularly in T-cell dependent B cell responses is anticipated. CD40 Ligand is expressed on the surface of activated CD4+ T cells, basophils, and mast cells. Binding of CD40L to its receptor, CD40, on the surface of B cells stimulates B-cell proliferation, adhesion and differentiation. A soluble isoform of CD40L has been shown to exist in the circulation. This soluble molecule is a homotrimer of a 18kDa protein exhibiting full activity in B cell proliferation and differentiation assays, is able to rescue B cells from apoptosis and binds soluble CD40. CD40L is discussed in relation to a potential role in supporting B cell tumors and it has been discovered that the molecular defect in the X-linked Hyper-IgM-Syndrome is targeted to the CD40 L gene, it is functional involved in B cell hybridomas and chronic lymphocytic leukemia as well as several autoimmune diseases.

Alternate Names

CD154, CD40L, CD40LG, gp39, TNFSF5, TRAP

Gene Symbol

CD40LG

Additional CD40 Ligand/TNFSF5 Products

Product Documents for Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human CD40 Ligand/TNFSF5 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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