Human CD55/DAF ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80311

Novus Biologicals, part of Bio-Techne
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NBP2-80311
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Key Product Details

Sample Type & Volume Required Per Well

Serum (100 ul)

Sensitivity

91.65 pg/ml

Assay Range

125-8000 pg/ml (example only; lot dependent)

View all CD55/DAF ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Conjugate

HRP

Reactivity

Human

Specificity

Recognizes both recombinant and natural Human CD55/DAF.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 3

Inter-Assay Precision (Precision between assays) CV% < 3.6 %

Recovery for Human CD55/DAF ELISA Kit (Colorimetric)

Recovery

82 -86%

Scientific Data Images for Human CD55/DAF ELISA Kit (Colorimetric)

ELISA: Human CD55/DAF ELISA Kit (Colorimetric) [NBP2-80311]

ELISA: Human CD55/DAF ELISA Kit (Colorimetric) [NBP2-80311]

ELISA: Human CD55/DAF ELISA Kit (Colorimetric) [NBP2-80311] - Example standard curve for Human CD55/DAF ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Kit Contents for Human CD55/DAF ELISA Kit (Colorimetric)

  • 1. 96 well microplate coated with Capture Antibody
  • 2. Detection Antibody conjugated to HRP
  • 3. Standards
  • 4. Wash Buffer Concentrate
  • 5. Dilution Buffer Concentrate
  • 6. Color Reagent A
  • 7. Color Reagent B
  • 8. Stop Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage is content dependent.

Background: CD55/DAF

CD55 encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Alternate Names

CD55, CR, DAF, TC

Gene Symbol

CD55

Additional CD55/DAF Products

Product Documents for Human CD55/DAF ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human CD55/DAF ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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