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Human CEBP gamma ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-75219

Novus Biologicals, part of Bio-Techne
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NBP2-75219

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

1.88 ng/mL

Assay Range

3.13 - 200 ng/mL

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This kit recognizes Human C/EBP(gamma) in samples. No significant cross-reactivity or interference between Human C/EBP(gamma) and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 4.72%

Inter-Assay Precision (Precision between assays) CV% < 4.83%

Recovery for Human CEBP gamma ELISA Kit (Colorimetric)

Recovery

85-105%

Linearity

Human CEBP gamma ELISA Kit (Colorimetric)

Scientific Data Images for Human CEBP gamma ELISA Kit (Colorimetric)

Human CEBP gamma ELISA Kit (Colorimetric)

ELISA: Human CEBP gamma ELISA Kit (Colorimetric) [NBP2-75219] -

ELISA: Human CEBP gamma ELISA Kit (Colorimetric) [NBP2-75219] - Standard Curve Reference

Kit Contents for Human CEBP gamma ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: CEBP gamma

The transcription factor C/EBP alpha (CCAAT-enhancer binding protein) is a heatstable, sequence-specific DNA-binding protein first purified from rat liver nuclei that binds avidly to several different cis-regulatory DNA sequences commonly associated with viral and cellular genes transcribed by RNA polymerase II. C/EBP alpha regulates gene expression in a variety of tissues including liver, adipose, lung and intestine. C/EBP alpha uses a bipartite structural motif to bind DNA. Two protein chains dimerize through a set of amphipathic alpha helices termed the leucine zipper. Highly basic polypeptide regions emerge from the zipper to form a linked set of DNA contact surfaces. C/EBP alpha appears to function exclusively in terminally differentiated, growth-arrested cells. Additional family members include C/EBP beta, C/EBP gamma,C/EBP delta and C/EBP episilon, all of which exhibit similar DNA-binding specificities and affinities to C/EBP alpha. Furthermore, C/EBP beta and C/EBP delta readily form heterodimers both with each other as well as with C/EBP alpha.

Alternate Names

c/EBP gamma, CCAAT/enhancer binding protein (C/EBP), gamma, CCAAT/enhancer-binding protein gamma, GPE1BP, IG/EBP-1

Gene Symbol

CEBPG

Additional CEBP gamma Products

Product Documents for Human CEBP gamma ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human CEBP gamma ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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