Rat Complement C4 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70042

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

1.88 ng/mL

Assay Range

3.13 - 200 ng/mL

View all Complement C4 ELISA Kits »

Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

This kit recognizes Rat C4 in samples. No significant cross-reactivity or interference between Rat C4 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.17%

Inter-Assay Precision (Precision between assays) CV% < 5.18%

Recovery for Rat Complement C4 ELISA Kit (Colorimetric)

Recovery

86-109%

Linearity

Rat Complement C4 ELISA Kit (Colorimetric)

Scientific Data Images for Rat Complement C4 ELISA Kit (Colorimetric)

Rat Complement C4 ELISA Kit (Colorimetric)

ELISA: Rat Complement C4 ELISA Kit (Colorimetric) [NBP2-70042] -

ELISA: Rat Complement C4 ELISA Kit (Colorimetric) [NBP2-70042] - Standard Curve Reference

Kit Contents for Rat Complement C4 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Complement C4

C4 consists of three polypeptide chains (alpha, molecular weight 95 kDa, beta, molecular weight 75 kDa and gamma, molecular weight 33 kDa) and is usually present in serum at 200-600mg/L. It is a protein of the classical complement pathway. When activated by C1s, a peptide C4a is released and the generated C4b binds C2 which is in turn cleaved by C1s to produce the classical pathway C3 convertase, namely C4b2a. C4b also has opsonin activity and binds to macrophages and neutrophils via their CR1 receptor.

Alternate Names

C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, C4, complement component 4A (Rodgers blood group), RG

Gene Symbol

C4A

Additional Complement C4 Products

Product Documents for Rat Complement C4 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat Complement C4 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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