Human, Monkey Complement C5 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # KA2114

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell Culture Samples, CSF, Milk, Plasma, Saliva and Serum (50 uL)

Sensitivity

53 pg/mL

Assay Range

0.156 to 10 ng/mL

View all Complement C5 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human, Monkey

Description

C5 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human C5.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 31.%

Inter-Assay Precision (Precision between assays) CV% < 9.9%

Recovery for Human, Monkey Complement C5 ELISA Kit (Colorimetric)

Recovery

98.5%

Scientific Data Images for Human, Monkey Complement C5 ELISA Kit (Colorimetric)

ELISA: Human, Monkey Complement C5 ELISA Kit (Colorimetric) [KA2114]

ELISA: Human, Monkey Complement C5 ELISA Kit (Colorimetric) [KA2114]

ELISA: Complement C5 ELISA Kit [Biotin] [KA2114] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.

Kit Contents for Human, Monkey Complement C5 ELISA Kit (Colorimetric)

  • Biotinylated Human Complement C5 Antibody (50x): A 50-fold concentrated biotinylated polyclonal antibody against human C5.
  • Chromogen Substrate (1x): A stabilized peroxidase chromogen substrate tetramethylbenzidine.
  • Human Complement C5 Microplate: A 96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against human C5.
  • Human Complement C5 Standard: Human C5 in a buffered protein base, lyophilized.
  • MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base.
  • Sealing Tapes: Pre-cut, pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay.
  • SP Conjugate (100x): A 100-fold concentrate.
  • Stop Solution (1x): A 0.5 N hydrochloric acid solution to stop the chromogen substrate reaction.
  • Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Complement C5

C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190kDa glycoprotein consisting of a disulphide linked alpha-chain (115kDa) and beta-chain (75kDa). C5 occurs in plasma at about 75mg/L. It is activated via either the classical or the alternative complement pathway, by protoelytic cleavage of C5a (11.5kDa) from the N-terminal of the alpha-chain. C5a is a potent anaphylotoxin and stimulates directed migration of neutrophils, eosinophils, basophils and monocytes. The other fragment, C5b,initiates the assembly of the membrane attack complex that mediates the cytolysis of viral and bacterial pathogens.

Alternate Names

anaphylatoxin C5a analog, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4, C5, complement C5, complement component 5, CPAMD4MGC142298, FLJ17816, FLJ17822

Gene Symbol

C5

Additional Complement C5 Products

Product Documents for Human, Monkey Complement C5 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human, Monkey Complement C5 ELISA Kit (Colorimetric)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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